Digital holographic microscopy at Wikipedia

The LISST Holo has been integrated with Dorado

Jericho et al describe a submersible digital inline holographic microscope (SDIHM), with ~2 micron spatial resolution and subsecond temporal resolution. Rated to 20 meters depth. A deep version rated to 6000 meters, with ~5 micron resolution is described by Bochdansky et al. Spatial resolution is considerably higher than LISST Holo (~25 microns). Video presentation; describes lightweight 2 kg version for arctic app. A version of this instrument is sold by Resolution Optics.

• Simplicity: microscopy without lenses; laser + pinhole + CCD camera
• Maximum information: single hologram contains all 3D structural information
• Maximum resolution: "optimal" resolution "easily" attainable - down to level of bacteria
• Speed kinetics of motion/reactions can be followed at video rate in 3D

Resolution Optics might lend a Submersible Microscope to MBARI for evaluation.

SubmersibleHolographicMicroscope.pdf (application/x-pdf)
DeepDIHM.pdf (application/x-pdf)